Pipette 23.6.13 instal the last version for windows

The neocortex develops in an inside-out manner, with principal cells in superficial layers migrating past earlier-born neurons in deeper layers 10. Pyramidal neurons (PNs) in different cortical layers differ in their expression of molecular markers 1, 2, 3, responses to sensory stimuli 4, patterns of synaptic connectivity 5, and morphological properties 6, 7, 8, 9. Our characterisation of the early development and maturation of pyramidal neurons in mouse mPFC not only demonstrates a comparable time course of postnatal maturation to that in other neocortical circuits, but also implies that consideration of layer- and time-specific changes in pyramidal neurons may be relevant for studies in mouse models of neuropsychiatric and neurodevelopmental disorders. In contrast, the balance of synaptic excitation and inhibition differs in a layer-specific pattern from one to four postnatal weeks of age. Our data demonstrate similar maturation profiles of dendritic morphology and intrinsic properties of pyramidal neurons in both deep and superficial layers. Here, we provide a detailed morphological and functional characterisation of pyramidal neurons in mouse mPFC during the first postnatal month, corresponding to known critical periods for synapse and neuron formation in mouse sensory neocortex. While the differences between principal cells across layers have been extensively studied, it is not known how the developmental profiles of neurons in different layers compare. Each layer is populated by distinct subtypes of principal cells that are born at different times during development. Mammalian neocortex is a highly layered structure.

Pipette 23.6.13 instal the last version for windows